Quantitative analysis of DNA damage and repair activities will form an essential part of genome stability research. As no single lab can master all the sophisticated technologies involved, a service platform will be established to provide access for all network members to a range of important tools, including quantification of strand breaks by comet assays, detection of damage and repair foci by automated high-resolution microscopy, detection of DNA adducts by immunohistochemistry and South-Western blotting, functional assays for specific DNA repair pathways and replication fork movement, and cytogenetic analyses for quantifying chromosomal aberrations and recombination events. The platform will also curate a database of reagents and protocols to promote standardisation of experiments in the network.
DNA damage & repair assays
