In this project, we aim to systematically investigate the role of different chromatin remodelling complexes and their components in genome protection and repair, with a focus on DNA double-strand breaks (DSBs). We will perform phenotype screening and genome-wide mapping of DSBs in human cell line models with complex subunit perturbations and combine this with proteomics approaches. We will furthermore integrate various published and own 'omics data resources from humans and yeast to identify potential functional relationships and dependencies between chromatin remodelling and genome stability factors. These dependencies will be followed up experimentally and represent potential routes for the therapy of related cancers.
Systematic investigation of the role of chromatin remodelling complexes in genome stability