Project 6 - Cristina Cardoso

This project aims at elucidating how the DNA replication machinery reacts to DNA lesions. We have generated a large collection of engineered human and mouse cells with labeled components of the replication machinery as well as sensors for ubiquitin modifications in live cells. Using these models and a combination of microscopical and genomics approaches, we will generate functional and kinetic spatio-temporal models of the response of the genome replication machinery to damages and how cellular physiology is affected.