This project aims at elucidating how DNA lesions are processed during genome replication. We have developed a method for the high-resolution mapping of DNA single-strand breaks and base lesions and will now implement additional features to this tool to enhance its versatility. In combination with a set of affinity probes selective for the pathway of ubiquitin-dependent DNA damage tolerance, this approach will serve to detect relevant regions of the genome where DNA damage bypass is important and to identify critical factors that provide information about the mechanism of damage processing.
Prof. Dr Helle Ulrich
Institute of Molecular Biology (IMB)
Webpage Ulrich lab
Publications since 2019
Project summary first funding period (2019-2022)