New review by the Stingele lab on transcription-coupled repair of DNA-protein crosslinks
Carnie CJ, Jackson SP, Stingele J (2024) Transcription-coupled repair of DNA-protein crosslinks.Trends Cell Biol., doi: 10.1016/j.tcb.2024.11.003 Link
Abstract:
DNA–protein crosslinks (DPCs) are highly toxic DNA lesions that are relevant to multiple human diseases. They are caused by various endogenous and environmental agents, and from the actions of enzymes such as topoisomerases. DPCs impede DNA polymerases, triggering replication-coupled DPC repair. Until recently the consequences of DPC blockade of RNA polymerases remained unclear. New methodologies for studying DPC repair have enabled the discovery of a transcription-coupled (TC) DPC repair pathway. Briefly, RNA polymerase II (RNAPII) stalling initiates TC-DPC repair, leading to sequential engagement of Cockayne syndrome (CS) proteins CSB and CSA, and to proteasomal degradation of the DPC. Deficient TC-DPC repair caused by loss of CSA or CSB function may help to explain the complex clinical presentation of CS patients.
Read the full paper here: https://www.sciencedirect.com/science/article/pii/S0962892424002344?via%3Dihub
