New publication from the Ulrich lab on the detection of DNA damage bypass by means of avidity-based biosensors

New publication in Science Advances from the Ulrich lab that describe the design, validation, and application of a series of avidity-based probes against the ubiquitylated forms of the DNA replication clamp, proliferating cell nuclear antigen (PCNA), in budding yeast. The approach is applicable to a wide range of ubiquitin-conjugated proteins, thus representing a generalizable strategy for the design of biosensors for specific (poly)ubiquitylated forms of individual substrates.

Read the full paper here:

Yakoub G, Choi Y-S, Wong RP, Strauch T, Ann KJ, Cohen RE and Ulrich HD (2023) Avidity-based biosensors for ubiquitylated PCNA reveal choreography of DNA damage bypass. Sci Adv, doi:10.1126/sciadv.adf3041